Date: Wed, 22 Jan 1997 09:36:56 -0600
Message-Id: <v01530507af0ce22f3dfc@[128.252.197.88]>
To: abrfhyp@cco.caltech.edu
From: rskubish@pharmdec.wustl.edu (Richard Skubish)
Subject: Recovery of lyophilized peptide
>Comments: Authenticated sender is <titov@ibch.siobc.ras.ru>
>From: "Vladimir Titov" <titov@ibch.siobc.ras.ru>
>Organization: Shemyakin-Ovchinnikov Institute of bioorgan
>Old-To: abrf@aecom.yu.edu
>Date: Thu, 17 Oct 1996 12:54:07 +0300
>Mime-Version: 1.0
>Subject: Re: Recovery of lyophilized peptide
>Priority: normal
>To: Recipients of ABRF List <abrf@aecom.yu.edu>
>Sender: Association of Biomolecular Resource Facilities
><abrf-request@aecom.yu.edu>
>Precedence: bulk
>
>> We have a customer who is getting poor recovery of a hydroscopic peptide
>> (approximately 15 residues) upon lyophilization. Any suggestions to improve
>> recovery?
>>
>> Stephen W. Coates
>> Rockland Technologies, Inc.
>
>Many peptides tend to form light powders and fly with the vapor upon
>lyophilization. The best cure is to use a speed vac concentrator; if you
>haven't one, seal your flask or adapter with parafilm and make small holes
>with a pin or so. Parafilm will trap the substance's particles
>
>Vladimir Titov
>MailTo:titov@ibch.siobc.ras.ru
>http://titov.siobc.ras.ru
>
Richard Skubish
rskubish@pharmdec.wustl.edu
314-362-0283
Washington University Medical School
Box 8103 - PNACL
660 S. Euclid
St. Louis, MO 63110