Date: 22 Jan 97 17:24:15 EST
From: Stephen.A.Bobin@dartmouth.edu (Stephen A. Bobin)
Subject: keratin
To: Recipients of ABRF List <abrf@aecom.yu.edu>
I just seqenced a couple of peptides from a tryptic digest of a PVDF blot. The
sequences the "customer" wanted were at about the 10-15 pmole level. In one of
the peptides a second sequence starting at about the 3 pmole level was easily
read out to twelve amino acids. This second sequence turned out to be a
tryptic peptide from keratin. So my question is where did it come from, skin?
As I told the customer "I wore gloves".
I recall seeing this a couple of years ago and chalked it up to someone's poor
technique and forgot about it.
I wonder how often this happens?
Has any one sniped off a chunk of skin and processed it for internal
sequencing?
Steve