Date: Thu, 23 Jan 1997 16:38:02 +0100
Message-Id: <199701231538.QAA22018@strix.its.uu.se>
From: Ulf Hellman <Ulf.Hellman@LICR.uu.se>
Subject: Sparging for HPLC
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Hi Adam,
I do not know if you have a very particular type of samples, but for my
types, peptides and proteins, nowadays in the low microgram range, I have
not bothered about any sparging for 10 years. The problems with out-gassing
have been conveniently solved by a flow restrictor AFTER the UV-cell, giving
just enough back pressure to keep gases in solution. I haven't even
de-aerated my solvents. For the Beckman Gold, I had one little gadget made
that simply squeezed the outlet teflon tubing to give some 3-4 bars. The
Smart pumps have a restrictor built in. Then again, maybe some pump makes
require sparging...
/Ulf
Ulf Hellman
Ludwig Institute for Cancer Research
Box 595, S-751 24 Uppsala Sweden
Phone: +46 18 160423 Fax: +46 18 160420 E-mail: ulf.hellman@licr.uu.se