From: GSCAVEY@am.pnu.com
Date: Thu, 23 Jan 1997 14:15:18 -0500
Message-Id: <00008CD9.1444@am.pnu.com>
Subject: ProtSeq:Feasibility of MS Detection for Edman Sequencer
To: Recipients of ABRF List <abrf@aecom.yu.edu>
ABRF, Grey Beards, White Hairs, Mass Spectrometrists, Lurkers, Whomever,
In light of the upcoming ABRF meeting and the workshop "Edman Chemistry in
the Next Millennium," I would like to ask the ABRF community for thoughts
on whether coupling a mass spectrometer to an Edman Sequencer will be a
benefit to exploiting the genome-proteome interface?
I would be interested in references for Edman sequencing with MS detection
and comments related to technical challenges with this approach. Is the
potential major advantage sensitivity or are there any advantages in speed
or disadvantages in selectivity? i.e. Leu/Ile What would be a
state-of-the-art MS system for such an application? Does the availability
of a high sensitivity-low flow cLC Procise sequencer help advance this
technology or can existing sequencer HPLC's with flow splitting be used?
Thank you in advance for your thoughts,
Gregory S. Cavey
Pharmacia & Upjohn