Date: Thu, 23 Jan 1997 22:38:16 GMT
Message-Id: <v02140b05af0d238e2a7e@[134.197.50.216]>
From: schooley@unr.edu (David A. Schooley)
Subject: Re: Sparging for HPLC
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Adam-
I thought all high purity gas regulators used stainless steel
diaphragms! Regular regulators (about half the price) have polymer
diaphragms, viton or something similar. These polymers bleed organic
materials into the water and give rise to contaminant peaks. This is most
definitely a problem if you intend to sparge. I thought teflon was far to
helium permeable to use in this way.
>I am setting up an HPLC, and I need to decide if a teflon lined regulator
>for high purity helium is a necessity. Has anyone ever heard of a
>regulator, or low purity helium for that matter contaminating their
>buffers?
>
David
David A. Schooley
Dept. of Biochemistry/330
Univ. of Nevada
Reno, NV 89557
schooley@med.unr.edu
tel: (702) 784-4136; fax (702) 784-1419