Date: Fri, 24 Jan 1997 09:18:35 +1100
From: Ken Mitchelhill <Ken_Mitchelhill@muwayf.unimelb.edu.au>
Subject: Re: 2D-gel storage
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Reply to: RE>2D-gel storage
Ruth,
as an exercise some years ago, we went down to our archives in the basement
and pulled out some (at least)10 year old gels out of some old lab workbooks
that had been stored in our "standard" way of drying them between two sheets
of cellophane, usually direct from destain solution. We were interested if
storage affected our ability to sequence from in-gel digests. We cut out some
of the standard proteins and compared them with similar intensity standards
from fresh gels and got similar yields and peptide profiles.
This is the way we tell all our investigators to store their gels.
Kindest regards...Ken
***
Ken Mitchelhill
The John Holt Protein Structure Laboratory
St. Vincent's Institute for Medical Research
41 Victoria Parade, Fitzroy 3065 Australia
Voice (03) 9288 2497 Fax (03) 9416 2676
ken_mitchelhill.medicine@muwayf.unimelb.edu.au
Maintainer of WWW home page for:
The Association of Biomolecular Resource Facilities
http://www.medstv.unimelb.edu.au/ABRF.html
***
--------------------------------------
Date: 24/01/1997 7:00 AM
To: Ken Mitchelhill
From: Ruth Hogue Angeletti
What is the best method of storage for 2D gels that one might one to keep
for an extended period of time for in-gel digestions?
Thanks for your experience,
Ruth
Ruth Hogue Angeletti
Albert Einstein College of Medicine
1300 Morris Park Avenue
tel: 718-430-3475
fax: 718-430-8939
angelett@aecom.yu.edu
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Date: Thu, 23 Jan 1997 12:13:32 -0500 (EST)
From: Ruth Hogue Angeletti <angelett@aecom.yu.edu>
Subject: 2D-gel storage
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