Date: Fri, 24 Jan 1997 11:32:37 +1100
From: Ken Mitchelhill <Ken_Mitchelhill@muwayf.unimelb.edu.au>
Subject: Re: ProtSeq-Feasibility of M
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Reply to: RE>ProtSeq:Feasibility of MS Detection for Edman Sequencer
Gregory,
at my Institute, where Pehr Edman was Director and the first automated
spinning cup sequenator was built by he and Geoff Begg, we have an annual
oration in Edman's honour. Three or four years ago, the guest lecturer was
Leroy Hood who actually showed slides of an Edman type sequencer with MS
detection that he was developing in his laboratory, at the time it sounded
like it was quite a way along the development path. I don't know if any data
from this instrument has ever been published, been expecting it to show up in
the literature or at a conference but I have read nor seen anything since.
I hope this is of interest...Ken
***
Ken Mitchelhill
The John Holt Protein Structure Laboratory
St. Vincent's Institute for Medical Research
41 Victoria Parade, Fitzroy 3065 Australia
Voice (03) 9288 2497 Fax (03) 9416 2676
ken_mitchelhill.medicine@muwayf.unimelb.edu.au
Maintainer of WWW home page for:
The Association of Biomolecular Resource Facilities
http://www.medstv.unimelb.edu.au/ABRF.html
***
--------------------------------------
Date: 24/01/1997 10:38 AM
To: Ken Mitchelhill
From: GSCAVEY@am.pnu.com
ABRF, Grey Beards, White Hairs, Mass Spectrometrists, Lurkers, Whomever,
In light of the upcoming ABRF meeting and the workshop "Edman Chemistry in
the Next Millennium," I would like to ask the ABRF community for thoughts
on whether coupling a mass spectrometer to an Edman Sequencer will be a
benefit to exploiting the genome-proteome interface?
I would be interested in references for Edman sequencing with MS detection
and comments related to technical challenges with this approach. Is the
potential major advantage sensitivity or are there any advantages in speed
or disadvantages in selectivity? i.e. Leu/Ile What would be a
state-of-the-art MS system for such an application? Does the availability
of a high sensitivity-low flow cLC Procise sequencer help advance this
technology or can existing sequencer HPLC's with flow splitting be used?
Thank you in advance for your thoughts,
Gregory S. Cavey
Pharmacia & Upjohn
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Date: Thu, 23 Jan 1997 14:15:18 -0500
From: GSCAVEY@am.pnu.com
Subject: ProtSeq:Feasibility of MS Detection for Edman Sequencer
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