Date: Fri, 24 Jan 1997 08:37:46 -0500 (EST)
From: Mark Lively <lively@mgrp.bgsm.edu>
Subject: Re: keratin
To: Recipients of ABRF List <abrf@aecom.yu.edu>
On Thu, 23 Jan 1997, Jacek Mozdzanowski-1 wrote:
> One of the most common sources of keratin(?) contamination is buffer B (1M
> tris, pH 6.7) used for the preparation of stacking gel of the standard
> tris/glycine polyacrylamide gels. We routinely keep the buffer refrigerated
> for no longer then 4 weeks - then discard it and prepare fresh. Our buffer B
> contains 0.05% azide. If the buffer goes wrong then there is a double band
> across the gel visible after silver staing of the gel or colloidal gold
For years we have observed a doublet of contaminant proteins in the range
of 55 to 60 kDa (?) but have never agreed upon the identity or source.
Is this the size of the double band that you are discussing? It would be
nice to finally give them a name and know where to look to eliminate
their presence!
ml
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Mark O. Lively, Ph.D. Voice: 910-716-2969
Professor of Biochemistry Fax: 910-716-7671
Bowman Gray School of Medicine email: Lively@mgrp.bgsm.edu
Wake Forest University
Winston-Salem, NC 27157
Home page: http://www.bgsm.edu/molecular_genetics/
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