Message-Id: <s2e8d024.043@genetics.com>
Date: Fri, 24 Jan 1997 15:13:42 -0500
From: Elliott Nickbarg <ENickbarg@genetics.com>
Subject: Re:ProtSeq:Feasibility of MS Detection for Edman Sequencer
To: Recipients of ABRF List <abrf@aecom.yu.edu>
I think that an Edman sequencer coupled to an MS detector would have
some advantages in addition to sensitivity. One could potentially do
away with the need to do chromatography, since one would identify
residues by their mass only. This would also reduce cycle times. A
disadvantage is that one loses the ability to distinguish between
Ile/Leu and Q/K if one eliminates the chromatography. Although I'm
not an instrument builder, I bet that an Edman/ms using an el cheapo
ion trap detector could do the trick at probably the same price (or
less) as a conventional Edman microsequencer.
Ruedi Aebersold published some stuff on PTH derivitives that were
designed to enhance ESI-ms detection. I don't know what the status
is of this research.
Refs:
Hess, D., Nika, H., Chow, D.T., Bures, E.J., Morrison, H.D.,
and Aebersold, R.
Liquid chromatography-electrospray ionization mass
spectrometry of 4-(3-pyridinylmethylaminocarboxypropyl)
phenylthiohydantoins. Analytical
Biochemistry 1995 Jan 1. 224(1). P 373-81.
Bures,E.J., Nika,H., Chow,D.T., Morrison,H.D., Hess,D., and
Aebersold,R.
Synthesis of the protein-sequencing reagent 4-(3-
pyridinylmethylaminocarboxypropyl) phenyl isothiocyanate and
characterization of
4-(3-pyridinylmethylaminocarboxypropyl) phenylthiohydantoins.
Analytical Biochemistry 1995 Jan 1. 224(1). P 364-72.
Yours,
Elliott Nickbarg
Genetics Inst., Inc.