Message-Id: <v03007812af13e975bd19@[131.215.234.49]>
Date: Tue, 28 Jan 1997 10:36:09 -0700
To: "abrfhyp@cco.caltech.edu" <abrfhyp@cco.caltech.edu>
From: "Dirk S. Krapf" <krapf@cco.caltech.edu>
Subject: HPLC resins for labile proteins (fwd)
Date: Sat, 25 Jan 1997 21:42:59 -0500 (EST)
From: Steven Birken <birks@styx.ios.com>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Subject: HPLC resins for labile proteins
What has been the experience of investigators with HPLC type
resins and columns for labile proteins for micro-scale purifications?
I have not found equivalents for e some of the Pharmacia ion
exchangers that are available for very small scale as the Smart
System. I have some ABI brownlee 2.1mm x 3cm anion and cation
"polypore" columns which use non-silica supports. Does anyone know
how these columns perform with labile proteins in terms of
acitivity recovery? I do not think silica based columns allow
recovery of activity of labile growth factors.