Message-Id: <n1357507480.83971@amgen.com>
Date: 30 Jan 1997 08:50:17 -0800
From: "Chris Clogston" <Chris.Clogston@amgen.com>
Subject: Re: HP-Seq Artifact Peak
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Reply to: RE>HP-Seq Artifact Peak
Steve,
We have it on all of our HP sequencers at Amgen. Our service engineer contacted people at the factory and they have no indication of a problem. We are also seeing it only in out 3.5 chemistry, so I suspect the toluene containing solvents.
Chris Clogston
Protein Structure
(805) 447-3748
(805) 499-7464 (fax)
Chris.Clogston@amgen.com
--------------------------------------
Date: 1/29/97 10:05 PM
To: Chris Clogston
From: StvTindall@AOL.com
Dear G1005A-ers,
We are getting a very broad peak which extends from Thr through Gly on
our HP sequencer. It is only present when using 3.5 chemistry. It
integrates as 0.5 to 2 pmol of Gln and tends to grow during a run. Its
appearance did not correlate exactly with any reagent changes, but S2/3 was
changed the day before the artifact started jumping. The lot numbers for
S2/3 and S3 are 31-0097-02 and 24-3266-04, respectively. We would try
different lot numbers, but we just have more of the same.
Is anyone else experiencing this problem? Is anyone using the same S2/3
and S3 lots and not getting the artifact?
Many thanks in advance.
Steve
====================
Stephen Tindall
Argo BioAnalytica, Inc.
Phone: 1-201-605-2100
Fax: 1-201-605-2104
StvTindall@aol.com
====================
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Date: Wed, 29 Jan 1997 22:58:28 -0500 (EST)
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Subject: HP-Seq Artifact Peak
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