Message-Id: <v01530504af16cd9a1fa0@[129.85.13.54]>
Date: Thu, 30 Jan 1997 17:27:24 -0500
From: fernaj@rockvax.rockefeller.edu (Joe Fernandez)
Subject: Re: HP-Seq Artifact Peak
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Steve,
We are using lot number 24-3266-04 of S3 and everything appears
normal. We have never seen the contaminant peak your decribing so it could
be something unusual. Best of luck and see you Baltimore.
Joe
>Dear G1005A-ers,
>
> We are getting a very broad peak which extends from Thr through Gly on
>our HP sequencer. It is only present when using 3.5 chemistry. It
>integrates as 0.5 to 2 pmol of Gln and tends to grow during a run. Its
>appearance did not correlate exactly with any reagent changes, but S2/3 was
>changed the day before the artifact started jumping. The lot numbers for
>S2/3 and S3 are 31-0097-02 and 24-3266-04, respectively. We would try
>different lot numbers, but we just have more of the same.
>
> Is anyone else experiencing this problem? Is anyone using the same S2/3
>and S3 lots and not getting the artifact?
>
>Many thanks in advance.
>
>Steve
>====================
>Stephen Tindall
>Argo BioAnalytica, Inc.
>Phone: 1-201-605-2100
>Fax: 1-201-605-2104
>StvTindall@aol.com
>====================