Date: Fri, 31 Jan 1997 08:28:41 -0700 (MST)
From: John Stewart <John.Stewart@uchsc.edu>
Subject: Re: PepSyn: Antibodies to 7-TM protein
In-Reply-To: <199701302205.AA22849@miwr03il.wh.bayer.com>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Werner Muller-Esterl and his group have raised antibodies
against various parts of the bradykinin B2 receptor, and these have been
used by various groups in a variety of studies. See Abd Alla et al., JBC
271:1748, 1996; Biochemistry 35:7514, 1996, as well as various papers in
Immunopharmacology vol. 33, 1996
John M. Stewart, Department of Biochemistry
Univ. of Colorado Medical School, Denver, CO 80262
Phone: 303-315-7534; FAX: 303-315-8215
Email: John.Stewart@UCHSC.edu
On Thu, 30 Jan 1997 gary@wh.bayer.com wrote:
> I've heard that it can be difficult to generate polyclonal
> antibodies to peptides from any 7-transmembrane protein
> for the purpose of identifying that protein by Western blot.
> Has anyone observed this problem? If so, do you have any
> specific recommendations on the selection of peptides
> (e.g. long or short), comments on best carrier (KLH?), or
> other general hints specific to 7-transmembrane proteins?
> Have you been successful using peptides from short loop regions
> between the transmembrane segments?
>
> Gary Davis
> Bayer Corp.
>
>