Date: Mon, 03 Feb 1997 09:49:51 -0800
From: Marc Lemaitre <m.lemaitre@eurogentec.be>
Subject: Re: visible dyes for PAGE/Urea
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Winnell Newman wrote:
>
> Dear all:
>
> I am trying to locate a visible dye in the range of 20-50 base pairs to run
> on urea/PAGE gels...any suggestions of whom to contact?
>
> many thanks
>
> Winnell
>
> Winnell H. Newman Director, Molecular Genetics Facility
Dear Dr. Newman,
I would like to be sure that I understand correctly your question.
Do you want to visualize a specific fragment AFTER the run
OR
Do you want a dye that would migrate, together with the DNA fragments
with a migration rate equivalent to a 20-50 bp piece ?
Also I would like to know the percentage of Acrylamide in your gel.
To give anyway an answer, we are using in our DNA 7MUREA/PAGE 2dyes :
Bromophenol blue and Xylene Cyanol. In a 15% gel, the first si migrating
like a 43 base ss-DNA piece and the second like a 15mer.
I hope it may help.
Maybe see you soon in Baltimore.
-- Marc LEMAITRE, Ph.D. EUROGENTEC Bel S.A. Parc de Recherche Cense Rouge B-4102 SERAING (BELGIUM) Tel : (+ 32) 4 366 01 50 Fax : (+ 32) 4 367 45 00 E-mail : <mailto:m.lemaitre@eurogentec.be> Web site : <http://www.eurogentec.be> <-------- check it out !