PEPSYN: n-terminal formylation

Haaseth@brcf.med.umich.edu
Tue, 16 Sep 97 11:55:10 EST

Dear esteemed colleagues:

Can anyone please give me advice about synthesizing a peptide
with an N-formyl methionine on the N-terminus? Specifically,
after I attach N-formyl methionine (available from Sigma) is the
formyl group stable to TFA cleavage? Any other hints about work-up
and purification?

Thanks in advance for any help or suggestions.

Ron Haaseth
Univeristy of Michigan