> We have had an old 380A that has served as our off-instrument ammonia
> cleavage apparatus. However, we are beginning to begrudge the bench space
> it takes up. It seems to me all we'd need for a semi-automated system
> would be a syringe pump and some sort of manifold to support the columns
> and collection vials. Has anyone else worked out such a system, and what
> did you use? Or is such an apparatus commercially available? Thanks in
> advance for your thoughts.
>
> Mary Kay Dolejsi, Ph.D.
> marykay@fred.fhcrc.org
> phone: 206-667-4470
> fax: 206-667-6497
>
> Mail address:
> Fred Hutchinson Cancer Research Center
> 1100 Fairview Ave N. A1-162
> PO Box 19024
> Seattle, WA 98109-1024
>
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Hi Mary Kay,
We simply break the columns in half and dump the contents into the vials
that fit on the machines. Then we add ammonium hydroxide (we do one ml),
deprotect at 65C for one hour, let cool, and desalt over a NAP 10 column
from pharmicia.
Hope this helps
Write back if it doesn't.
Good Luck!
Christopher O Conway
Lab Coordinator DNA Analysis Facility
Genetics Resources CORE Facility
Johns Hopkins University
Baltimore, Maryland USA