Prot Seq: when isn't it logical to assume a 3 times load?
Thomas J Miller E328/247 695-1745 (MILLERTJ@ESVAX-A1.EMAIL.DUPONT.COM)
Fri, 19 Sep 1997 09:53:31 -0400 (EDT)
Hello fellow sequencers. I just had an experience where it was safe (in my
mind) to assume that by tripling a pvdf sample load from 2 to 6 tracks, I
would see a likewise triple initial yield. Well I didn't. I saw a doubling
of the yields in the first half of the sequencer run, then strangely,
the expected 3x amounts. The sample origins was an overexpression in E-coli.
The pvdf blot looked consistent across the sheet. I'm using a Beckman
LF3000 sequencer. Any comments or suggestions as to why I saw this effect
would be appreciated. Thank you. Regards, Tom
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Thomas Miller, Macromolecular Analysis, Protein Sequencing
Dupont Co., POB 80328, Wilmington, DE 19880
Voice 302-695-1745
email: Thomas.J.Miller@usa.dupont.com
Opinions are my own and not of my employer.
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