DNASeq:200 base pair dropout
procor@smtpgate.bcsew.edu
Tue, 23 Sep 97 12:32:26 -0600
Hello ABRF's, We are having some problems here and need help.We are
running sequencing gels using an ABI 373. Our gels have been failing
after 180-300 basepair reads. The lanes do not go black but the sample
appears smeared in the the lane. We have replaced all the reagents,
the glass plates, the well forming comb. We were able to run our pGem
stds. on another instrument but when we took our gel to the other
instrument the gel failed. We have had service out and the laser,
temp, watts appaer fine. This problem started abruptly about 3 weeks
ago. Our ABI service engineer told us about 200 basepair dropout. Has
any one alse seen this phenomenon? Any sugesstions would be greatly
appreciated.
Trudy Holyst procor@smtpgate.bcsew.edu
Trudy Holyst
Blood Reasearch Institute
Blood Center of SE WI
PO BOX 2178
Milwaukee WI 53201