Re: DNASeq:200 base pair dropout

Alex (alexwang@welchlink.welch.jhu.edu)
Tue, 23 Sep 1997 14:35:11 -0400 (EDT)

It sounds like you are running out of buffer. You will usually have nice
bands and then all lanes will just smear out when the instrument runs out
of buffer. Try checking the buffer chamber for worn out gaskets.

On Tue, 23 Sep 1997 procor@smtpgate.bcsew.edu wrote:

>
> Hello ABRF's, We are having some problems here and need help.We are
> running sequencing gels using an ABI 373. Our gels have been failing
> after 180-300 basepair reads. The lanes do not go black but the sample
> appears smeared in the the lane. We have replaced all the reagents,
> the glass plates, the well forming comb. We were able to run our pGem
> stds. on another instrument but when we took our gel to the other
> instrument the gel failed. We have had service out and the laser,
> temp, watts appaer fine. This problem started abruptly about 3 weeks
> ago. Our ABI service engineer told us about 200 basepair dropout. Has
> any one alse seen this phenomenon? Any sugesstions would be greatly
> appreciated.
>
> Trudy Holyst procor@smtpgate.bcsew.edu
>
> Trudy Holyst
> Blood Reasearch Institute
> Blood Center of SE WI
> PO BOX 2178
> Milwaukee WI 53201
>