Microwave hydrolysis

Bryan Dunbar (b.dunbar@abdn.ac.uk)
Fri, 26 Sep 1997 05:02:32 +0100

Kris Gevaert wrote:
>
> Hello,
>
> I would like to find out if anybody has some experience with partial acid
> hydrolysis of proteins combined with product analysis by mass spectrometry
> (MALDI in our case)?
> I am interested in the use of microwave heating for the acid hydrolysis,
> but I can't find much information in the recent literature. Can anybody
> give me references about the latter?
>
> Thank you in advance,
>
> Kris Gevaert
> Flanders Interuniversity Institute for Biotechnology (V.I.B.)
> Department of Medical Protein Chemistry
> Universiteit Gent
> K.L. Ledeganckstraat 35
> B-9000 Gent
> Belgium
>
> Telephone: -- 32 / 9 2 64 52 91
> Fax: -- 32 / 9 2 64 52 93 or 53 37
> e-mail: krgev@gengenp.rug.ac.be
Kris,
my colleague Ian Davidson wrote about this in chapter 13, page 125
of "Protein Sequencing Protocols" Humana press - ISBN 0 89603 353 8
(editor Bryan Smith). Ian also mentioned two references -

1. Chiou, S.-H. and Wang, K.-T. (1990) Current Research in Protein
Chemistry (Villafranca, J.J., ed.),Academic Press pp 3-10

2. Gilman, L.B. and Woodward, C. (1990) Current Research in Protein
Chemistry vol. 3 (Villafranca, J.J., ed.),Academic Press pp 23-26

I hope this is of help - if you cannot lay your hands easily on Protein
Sequencing Protocols; then I can xerox the relevant section and send it
to you.
Regards,
Bryan.