I wrote a paper on partial acid hydrolysis of peptides.
"Peptide fingerprints after partial acid hydrolysis: analysis by
matrix-assisted laser desorption/ionization mass spectrometry."
Rapid Commun Mass Spectrom 8(12), 1007-1010 (1994)
I hope this helps. Please feel free to contact me if you have any
questions.
Michael D Knierman
Eli Lilly and Co.
knierman@lilly.com
>Hello,
>
>I would like to find out if anybody has some experience with partial acid
>hydrolysis of proteins combined with product analysis by mass
spectrometry
>(MALDI in our case)?
>I am interested in the use of microwave heating for the acid hydrolysis,
>but I can't find much information in the recent literature. Can anybody
>give me references about the latter?
>
>Thank you in advance,
>
>Kris Gevaert
>Flanders Interuniversity Institute for Biotechnology (V.I.B.)
>Department of Medical Protein Chemistry
>Universiteit Gent
>K.L. Ledeganckstraat 35
>B-9000 Gent
>Belgium
>
>Telephone: -- 32 / 9 2 64 52 91
>Fax: -- 32 / 9 2 64 52 93 or 53 37
>e-mail: krgev@gengenp.rug.ac.be
>