Specificity of chymotrypsin

Kenneth Williams (Kenneth.Williams@yale.edu)
Thu, 25 Sep 1997 17:42:11 -0400

The product literature with our sequencing grade chymotrypsin states this
enzyme cleaves at Tyr, Phe, Trp and (at a lower rate) at Leu, Met, Ala, Asp
and Glu. Has anyone documented reasonable rates of cleavage at Asp/Glu?
Is there a good reference(s) that documents relative rates of cleavage at
different residues in intact, denatured proteins?

Thanks,
Ken Williams