We have been using an HP1050 for size exclusion, primarily with
0.1 M phosphate / 0.1 M NaCl. The system is equipped with an HP
autoinjector, and continuously washes the pump seals to avoid salt
precipitation problems. We routinely run at around 0.5 ml/min, with 30-40
minute runs, and monitor at 215 nm. I believe this would fit your specs.
We are careful to flush with water when not in use, but have been running
continuously for months without any problems. Let me know if you need
further info,
Karen De Jongh
On Thu, 25 Sep 1997, Kenneth Williams wrote:
> We would like to set up an automated HPLC gel filtration system and need
> advice on an HPLC pump and autoinjector that can operate continuously in
> aqueous buffers that will always contain non-volatile buffers and salts
> that will be in the range of 0.05 to 0.5 M. The flow rates are anticipated
> to be in the range of 0.05 -1.0 ml/min, pressures up to 2500 psi and total
> solvent volumes/run up to about 30-40 mls. A final requirement is the pump
> needs to be as pulseless as possible to minimize interference with the
> detectors that will be used. All/any advice on vendors/models (either
> direct to me at kenneth.williams@yale.edu or via general posting) will be
> greatly appreciated.
>
> Thanks for your help (again),
>
> Ken Williams
>
>