There is an old but still good review paper about "Enzymatic Cleavage of
Proteins"
by Keil B (pages 291-304) that appeared in Methods in Protein Sequence
Analysis,
edited by Marshall Elzinga (1982, Humana Press Clifton, NJ). According to
this paper
relative rates of cleavage by chymotrypsin are Phe (100), Trp (91), Tyr (85),
Leu (66), Met (36), His (36), Asn (15), Gln (13), Thr (11), and Lys (5). No
cleavage
at Asp and Glu was stated. My own experiences well agree with it. You can find
more references in this review article.
-Young Moo
>The product literature with our sequencing grade chymotrypsin states this
>enzyme cleaves at Tyr, Phe, Trp and (at a lower rate) at Leu, Met, Ala, Asp
>and Glu. Has anyone documented reasonable rates of cleavage at Asp/Glu?
>Is there a good reference(s) that documents relative rates of cleavage at
>different residues in intact, denatured proteins?
>
> Thanks,
> Ken Williams
____
Young Moo Lee
Director
Protein Structure Laboratory
University of California
Davis, California 95616-8656
916-752-4328 (voice)
916-752-8969 (fax)
ymlee@ucdavis.edu
http://pubweb.ucdavis.edu/Documents/psl/psl_pg.htm