PEP SYN: Photoaffinity Probes

Haaseth@brcf.med.umich.edu
Thu, 15 Jan 98 13:07:12 EST

Dear ABRFers:

OK, I have several questions:

What do you you like to use as a photoaffinty reagent
for peptides? What is commercially available; where do
you get it?

I see Bachem has p-azidoPhe available in both Fmoc and Boc
forms. Do you like to use this? If so, what wavelength
of light do you use to activate it?

Will the conditions (Chloramine T) for radioiodinating a
tyrosine residue do any damage to the p-azido (or other
appropriate) group before binding and subsequent
photoactivation?

Thanks in advance for any help you can offer.

Ron Haaseth
University of Michigan
verdin@umich.edu