Re: Misc- UV grade guanidine-HC (fwd)
Dirk S. Krapf (krapf@gilson.com)
Fri, 16 Jan 1998 10:02:09 -0800 (PST)
>---------- Forwarded message ----------
>Date: Tue, 13 Jan 1998 11:50:37 -0500 (EST)
>From: Mark Lively <lively@mgrp.bgsm.edu>
>To: Recipients of ABRF List <abrf@aecom.yu.edu>
>Cc: Recipients of ABRF List <abrf@aecom.yu.edu>
>Subject: Re: Misc- UV grade guanidine-HC
>
>Michael,
> Years ago the Walsh lab used ultrapure guanidine HCl to do gel
>filtration HPLC of peptide fragments and monitored the separation at 210
>nm. Yes, they ran 6 M guanidine in an old Varian 5000 series HPLC and it
>worked very nicely. Surprisingly, the HPLC survived for years even with
>this very abusive solvent system and the seaparations were very useful!
>They used Ultrapure guanidine from Heico Chemical Corp., a Division of
>Whittaker Corp., Delaware Water Gap, PA 18327. My phone number for them
>dates back to February 1985 so I am not even sure if they are still in
>this business but you might give it a try: 717-476-0353 (old!). At that
>time, the cost was $68 per 500 gm bottle and a 2-bottle minimuim order. If
>you find that they still sell this product perhaps you could post the
>information for the rest of us.
>Good luck,
>Mark
>
>
>On Mon, 12 Jan 1998, Michael Bodo wrote:
>
>> Misc: UV grade guanidine-HCl
>> Dear fellow netters,
>>
>> I need to obtain far UV spectra of proteins in 6 M guanidinium
>>hydrochloride. Is
>> anyone of you aware of a source of spectroscopy grade Gnd-HCl, that has
>>a low absorbance
>> between 220 and 230 nm?
>>
>> Thanks a lot!
>>
>> Michael Bodo
>> FibroGen Inc.
>> (650)829-7363
>> mbodo@fibrogen.com
>>
>>
>>
>>
>
>-------------------------------------------------------------------------
>Mark O. Lively, Ph.D. Voice: 910-716-2969
>Professor of Biochemistry Fax: 910-716-7200
>Wake Forest University School of Medicine email: lively@mgrp.bgsm.edu
>Medical Center Blvd.
>Winston-Salem, NC 27157
> HOME PAGE: http://www.bgsm.edu/molecular_genetics/
>--------------------------------------------------------------------------
>