pep synth - advanced chem tech instrment (fwd)
Dirk S. Krapf (krapf@gilson.com)
Fri, 16 Jan 1998 10:02:12 -0800 (PST)
>---------- Forwarded message ----------
>Date: Tue, 13 Jan 98 15:36:27 EST
>From: "Richard F. Cook" <cook@MIT.EDU>
>To: Recipients of ABRF List <abrf@aecom.yu.edu>
>Subject: pep synth - advanced chem tech instrment
>
>
>
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>Dear ABRFers,
>
>
>I would like to find out if there are any common modifications to Advanced
>Chem Tech multiple peptide synthesizer protocols that are useful in getting
>high quality peptides made on that instrument(s). I have heard from a
>couple of different labs that they 1.) double couple all cycles 2.) use a
>10 fold molar excess of amino acid over resin and 3.) don't make anything
>longer than ca. 20 mers.
>
>If anyone has any other advice, perhaps on a resin type that works well if
>that is a factor, I'd love to read it.
>
>
>Thanks in advance,
>
>Dick Cook
>