>to purchase the bzl protected fmoc-(po)ser from Novobiochem but they are
>backordered until March. Bachem sells an unprotected p-ser and Chemimpex
>sells another protection scheme with no cleavage condition suggestions.
I am pasting in a receipe from David S. King of HHMI Berkeley for
on-resion phosphorylation. He is a real guru of peptide synthesis. I
regret to say we did not do it this way, but used the Bachem material as
it appeared easier.
David Schooley
From: "David King" <dk@bosco2.berkeley.edu>
Date: Tue, 28 May 1996 12:18:44 -0700
To: schooley@equinox.unr.edu (David A. Schooley)
I do phosphorylations the dirt cheap way - use FmocSer where you want the
pSer, and phosphorylate on the beads: with Aldrich cheap unhindered
ditBu-N,N-diEt phosphoramidite + = amt Fisher ($70 for 5 grams!) tetrazole,
takes 1-3h rt, rinse, then oxidize trivalent phosphorus with dilute Aldrich
aq tBuOOH 1 h (no Met sulfoxide generated), all in NMP. H-Phosphonate
(M-16) is only byproduct. My method does take some extra time. You get
nonphos peptide as well. If HBTU works with the novabiochem deriv, DCC/HOBT
will work at least as well.
David A. Schooley
Dept. of Biochemistry/330
Univ. of Nevada
Reno, NV 89557
schooley@med.unr.edu
tel: (702) 784-4136; fax (702) 784-1419