Re: Deamidation (fwd)

Richard Laursen (laursen@bu.edu)
Fri, 16 Jan 1998 16:27:18 +0000

An article entitled "Deamidation of Asparaginyl Residues as a Hazard in
Experimental Protein and Peptide Procedures" that appeared in Analytical
Biochemistry, 42, 565 (1971), may be of help. The authors found that
deamidation of Asn occurs rather rapidly at pH 10, particularly in
phosphate buffer.

Richard Laursen
--------------------------------------
>Hello
>
>I'm looking for a simple deamidation method for small peptides at low and
>high pH to be used in conjunction with CZE. Any references for a reliable
>method ?. Thanks.

Richard A. Laursen
Department of Chemistry
Boston University
590 Commonwealth Ave.
Boston, MA 02215
Tel (617) 353-2491; FAX (617) 353-6466
email: <laursen@bu.edu>