FW: PepSyn-HF apparatus acid traps

Everson, Anita [PRI] (AEverson@prius.jnj.com)
Fri, 23 Jan 1998 12:17:24 -0500

Hello, Mary! While we don't have have an Immunodynamics HF apparatus,
we also use 2 traps. The first one is just plain water, which I
recommend that you use instead of an empty bottle because most of your
HF will be trapped in the water. This one gets changed with every HF.
The next trap is 5M KOH to which we add the indicator bromophenol blue.
When the indicator shows that the KOH is exhausted, we make up fresh
KOH. The water and old KOH is disposed of down the sink with plenty of
water. Check with your Health & Safety guys and see what they recommend
for disposal.

Anita Everson
Peptide Lab, RW Johnson PRI
La Jolla, CA
aeverson@prius.jnj.com

> ----------
> From: Mary Bower[SMTP:bower@biochem.purdue.edu]
> Sent: Thursday, January 22, 1998 9:49 AM
> To: Recipients of ABRF List
> Subject: PepSyn-HF apparatus acid traps
>
> Hello everyone!
>
> I was hoping someone could provide me with the specifics on the KOH
> traps
> on their HF apparatus. I need to know the molarity of the KOH
> solution
> and what needs to be in each of the traps. I have an immunodynamics
> apparatus and it has been my understanding that the first trap
> (closest to
> the apparatus) contain nothing, and the second trap contain a solution
> of
> KOH. My traps need to be cleaned and refilled with KOH. What is the
> best
> disposal method for the solution currently in the second trap??
>
> Thanks in advance for your help!
>
> Mary
>
> Mary Bower
> Instrument Specialist
> Purdue University
> Department of Biochemistry
> West Lafayette, IN 47907
>
> Phone (765)494-6540
> Fax (765)494-7897
> email; Bower@Biochem.purdue.edu
>
>