We had the instrument in our lab for a couple of month, but Dan Little from Sequenom was the one who pipetted the samples. The system is called the Autodrop system. The company is located in Nordestedt Germany. I'll call Dan to get an address. In our lab we have a probot from LC packing it spots directly HPLC fractions on our MALDI probe, right now we can pipet 90-100 nanoliter, but if we use a narrower bore capillary we could pipet less. A better person to ask question about the probot is Jean Pierre Saltzmann.
Amina
Amina S. Woods, Ph.D.
Johns Hopkins School of Medicine
725 N Wolfe St., Baltimore, MD 21205
Tel: (410) 614-4981, Fax: (410) 955-3420
E-mail: amina@welchlink.welch.jhu.edu
----------
From: Robert Moritz [SMTP:Robert.Moritz@ludwig.edu.au]
Sent: Saturday, January 24, 1998 12:06 AM
To: 'Amina'
Cc: Robert Moritz
Subject: RE: High Sensitivity Protein Identification
Hi Amina,
With regards to the microdrop pipette, could you tell me the
brand and supplier you obtained this from.
With best regards,
Robert.
Robert Moritz
Chief Research Officer
Joint Protein Structure Laboratory
Ludwig Institute For Cancer Research &
The Walter and Eliza Hall Institute of Medical Research
Royal Melbourne Hospital, Parkville,
Victoria, Australia 3050
Phn +61-3-9347-3155, Fax +61-3-9348-1925
Email: Robert.Moritz@ludwig.edu.au or Moritz@wehi.edu.au
> -----Original Message-----
> From: Amina [SMTP:amina@welchlink.welch.jhu.edu]
> Sent: Saturday, 24 January 1998 2:04
> To: Robert Moritz
> Subject: RE: High Sensitivity Protein Identification
>
> Ken:
>
> Last year we used a microdrop pipetor that can pipet nanoliter
> amounts of a premixed solution of peptide and matrix. The spots that
> were made were very small. Using a Kratos MALDI 4 without delayed
> extraction, we were able to see as little as 3 pmoles deposited, it
> was a mediocre spectrum, but we got good spectra and around 200
> resolution for the 10 atomoles deposited. I think that the secret of
> success is in making small sample spots.
>
> Amina
>
>
> Amina S. Woods, Ph.D.
> Johns Hopkins School of Medicine
> 725 N Wolfe St., Baltimore, MD 21205
> Tel: (410) 614-4981, Fax: (410) 955-3420
> E-mail: amina@welchlink.welch.jhu.edu
>
>
>
> ----------
> From: Kenneth Williams [SMTP:Kenneth.Williams@yale.edu]
> Sent: Thursday, January 22, 1998 4:00 PM
> To: Recipients of ABRF List
> Subject: High Sensitivity Protein Identification
>
> I'm looking for references/unpublished observations for
> extremely high
> sensitivity MS or MS/MS protein identification - at or below the low
> amol
> level - to help one of our users who would like to propose (in a grant
> application) identifying some proteins at very low levels. My guess
> is
> MALDI/peptide mass searching is the most sensitive approach but
> perhaps I
> am wrong. Any help would be appreciated.
>
> Thanks very much,
>
> Ken Williams
> Yale/Keck Labs