Re: detection of residual SDS.

L. Ericsson (ericsson@u.washington.edu)
Mon, 26 Jan 1998 14:36:03 -0800 (PST)


To evaluate different procedures for the removal of SDS from proteins,
Konigsberg (Methods in Enzymology Vol. 91 pg 254-259 1983) added to his
proteins 35S[SDS]. I don't know if you have the option of using
radioactive SDS, but it is one way to quantitate residual SDS.
-Lowell H. Ericsson

On Mon, 26 Jan 1998, Andrews, David wrote:

> Can anyone out there direct me to appropriate references describing the
> detection (and quantitation!) of residual SDS in protein preparations?
> Thanks!
>
>
> David W. Andrews, Ph.D.
> Principal Scientist, Bioanalytical Group
> Astra Research Center Boston
> 128 Sidney Street
> Cambridge, MA 02139
>
> 617-234-2608 (voice)
> 617-576-3030 (fax)
> e-mail: david.andrews@arcb.us.astra.com
>
>