I have two questions about the coupling reaction.
Does anyone have any suggestions about how long
the reaction time shall be, if you want to form
an ester bond or a peptide bond?
Can a stock solution of HBTU/HOBt/DIEA
in DMF be made, to which you add the amino acid
when you want to make the coupling step?
When you have added the amino acid, shall you let the
solution activate for a while before adding it to the
solid phase. If that's the case, how long should the
activating time be?
If you can make up the above mentioned stock solution,
does somebody know for how long it will be stable?
If there is any other information about the reagents that
you want to share, please do so.
Best regards Maria Thyboll
Maria Thyboll
Department of Analytical Chemistry
Royal Institute of Technology
Teknikringen 36
100 44 Stockholm
SWEDEN
Tel +46 8 7906410
Fax + 46 8 108425
Email k93_mtl@k.kth.se