I have a large glycosylated peptide with a high number of serine and a few
aparagine residues. I would like to determine the glycosylated residue.
Would sequencing cleave through a glycosylated site? Since there are many
serine residues making identification of the glycosylated site difficult,
could a carboxypeptidase be used to generate a peptide ending in the
glycosylated residue? Would a peptidase cleave through a glycosylated
residue? Any other suggestions would be greatly appreciated. Thanks in
advance.
Paul Motchnik
XOMA Corp.