Regards,
Robert Moritz
Joint Protein Structure Laboratory
Ludwig Institute For Cancer Research &
The Walter and Eliza Hall Institute of Medical Research
Royal Melbourne Hospital, Parkville,
Victoria, Australia 3050
> -----Original Message-----
> From: PMotchnik@AOL.com [SMTP:PMotchnik@AOL.com]
> Sent: Saturday, 31 January 1998 5:45
> To: Robert Moritz
> Subject: ProtSeq
>
> Dear ABRFers,
>
> I have a large glycosylated peptide with a high number of serine and
> a few
> aparagine residues. I would like to determine the glycosylated
> residue.
> Would sequencing cleave through a glycosylated site? Since there are
> many
> serine residues making identification of the glycosylated site
> difficult,
> could a carboxypeptidase be used to generate a peptide ending in the
> glycosylated residue? Would a peptidase cleave through a glycosylated
> residue? Any other suggestions would be greatly appreciated. Thanks
> in
> advance.
>
> Paul Motchnik
> XOMA Corp.