DNAsyn: purification of longmers

Mary Kay Dolejsi (marykay@fred.fhcrc.org)
Mon, 2 Feb 1998 13:19:30 -0800

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What is the "best" method for purifying full-length long oligomers- like 80
- 85mers? A researcher here wants to make such a longmer, and have it >95%
pure. Can solid-phase methods, like OPC really do this? Can IEX resolve at
this length? Gel electrophoresis and elution?

I appreciate any help you can give us. I know there was a recent
discussion about a 45mer, but has anyone been working with longmers?

Thanks in advance.

Mary Kay Dolejsi, Ph.D.
marykay@fred.fhcrc.org
phone: 206-667-4470
fax: 206-667-6497

Mail address:
Fred Hutchinson Cancer Research Center
1100 Fairview Ave N. A1-162
PO Box 19024
Seattle, WA 98109-1024

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