Re: DNAsyn: purification of longmers

Scottie Adams (psadams@cell-science.org)
Tue, 3 Feb 1998 11:40:49 -0500

At 1:19 PM -0800 2/2/98, Mary Kay Dolejsi wrote:
>What is the "best" method for purifying full-length long oligomers- like 80
>- 85mers? A researcher here wants to make such a longmer, and have it >95%
>pure. Can solid-phase methods, like OPC really do this? Can IEX resolve at
>this length? Gel electrophoresis and elution?
>
>I appreciate any help you can give us. I know there was a recent
>discussion about a 45mer, but has anyone been working with longmers?

Mary Kay

I have made quite a few 75-90 mers in the past year and we gel
purified them, soaked the gel piece and then used a Waters Sep-pak just as
recommended in the old ABI User Bulletin #13. This works quite well, but
if someone has an easier method - I would like to hear it. Scottie

Scottie Adams aka Pamela Scott Adams

Molecular Biology Core Facility
Adirondack Biomedical Research Institute, Inc.
(formerly the W. Alton Jones Cell Science Center)
10 Old Barn Road
Lake Placid, NY 12946
Phone: 518-523-1276
Fax: 518-523-1849
Email: scottie@cell-science.org
http://www.cell-science.org/mbcf