Re: High Sensitivity Protein Identification (fwd)
Dirk S. Krapf (krapf@gilson.com)
Wed, 4 Feb 1998 08:57:57 -0800 (PST)
>---------- Forwarded message ----------
>Date: Fri, 23 Jan 1998 10:44:42 -0500
>From: Axel Ducret <axel_ducret@merck.com>
>To: Recipients of ABRF List <abrf@aecom.yu.edu>
>Subject: Re: High Sensitivity Protein Identification
>
>Ken,
>
>As a grant's reviewer, I would seriously doubt of a project that would propose
>to do MS or MS/MS protein characterization at the low amol level or below.
>In my
>
>knowledge, low fmol level (this is about what you will be able to detect with
>silver staining on gel and western blotting ...) is probably the best you will
>achieve (1 fmol of a 20 kDa protein = 20 PICOgrams ...).Here is a small (and
>certainly not complete !) list of representative litterature:
>
>for MALDI-Tof:
>
>Caprioli RM, Farmer TB, Gile J. (1997) Molecular imaging of biological
>samples:
>localization of peptides and proteins
>using MALDI-TOF MS. Anal. Chem. 69(23) 4751-4760
>
>Jensen ON, Podtelejnikov AV, Mann M (1997) Identification of the components of
>simple protein mixtures by
>high-accuracy peptide mass mapping and database searching. Anal. Chem. 69(23)
>4741-4750
>
>Cordwell SJ, Basseal DJ, Humphery-Smith I. (1997) Proteome analysis of
>Spiroplasma melliferum (A56) and protein characterisation across species
>boundaries.Electrophoresis 18(8) 1335-1346
>
>Qin J, Fenyo D, Zhao Y, Hall WW, Chao DM, Wilson CJ, Young RA, Chait BT
>(1997) A
>
>strategy for rapid, high-confidence protein identification. Anal. Chem. 69
>(19)
>3995-4001
>
>Pappin DJ (1997) Peptide mass fingerprinting using MALDI-TOF mass
>spectrometry.
>Methods Mol. Biol. 64 165-173
>
>For CE and LC-MS/MS and nanoES-MS/MS:
>
>McCormack AL, Schieltz DM, Goode B, Yang S, Barnes G, Drubin D, Yates JR 3rd
>(1997) Direct analysis and identification of proteins in mixtures by LC/MS/MS
>and database searching at the low-femtomole level. Anal Chem 69(4) 767-776
>
>Davis MT, Stahl DC, Hefta SA, Lee TD (1995) A microscale electrospray
>interface
>for on-line, capillary liquid
>chromatography/tandem mass spectrometry of complex peptide mixtures. Anal.
>Chem.
>
>67(24) 4549-4556
>
>Tong D, Moritz RL, Eddes JS, Reid GE, Rasmussen RK, Dorow DS, Simpson RJ
>(1997)
>Fabrication of stable packed capillary reversed-phase columns for protein
>structural analysis. J. Prot. Chem. 16(5) 425-431
>
>Wilm M, Shevchenko A, Houthaeve T, Breit S, Schweigerer L, Fotsis T, Mann M
>(1996) Femtomole sequencing of proteins from polyacrylamide gels by
>nano-electrospray mass spectrometry. Nature 379(6564) 466-469
>
>Morris HR, Paxton T, Panico M, McDowell R, Dell A (1997) A novel geometry mass
>spectrometer, the Q-TOF, for
>low-femtomole/attomole-range biopolymer sequencing. J. Prot. Chem. 16(5)
>469-479
>
>
>Figeys D, Ducret A, Yates JR 3rd, Aebersold R. (1996) Protein
>identification by
>solid-phase microextraction capillary-zone electrophoresis microelectrospray
>tandem mass spectrometry. Nature Biotech.14 1579-1583
>
>Wheat TE, Lilley KA, Banks JF (1997) Capillary electrophoresis with
>electrospray
>
>mass spectrometry detection
>for low-molecular-mass compounds. J. Chromatogr. A 781(1-2) 99-105
>
>Valaskovic GA, Kelleher NL, McLafferty FW (1996) Attomole protein
>characterization by capillary electrophoresis-mass
>spectrometry. Science 273(5279) 1199-1202
>
>Figeys D, Ning Y, Aebersold R (1997) A microfabricated device for rapid
>protein
>identification by microelectrospray ion trap mass spectrometry. Anal. Chem.
>69(16) 3153-3160
>
>Xue Q, Foret F, Dunayevskiy YM, Zavracky PM, McGruer NE, Karger BL (1997)
>Multichannel microchip electrospray mass spectrometry. Anal. Chem. 69(3)
>426-430
>
>
>Hope that it helps
>
>Axel Ducret
>
>-------------------------------------------------------------------------------
>-
>-----------------------------------
>
>Axel Ducret, Ph.D.
>Senior Research Biologist
>Merck-Frosst Canada Inc.
>Dept. Biochemistry and Molecular Biology
>P.O. Box 1005
>Pointe-Claire-Dorval PQ H9R 4P8
>Canada
>
>tel. + (514) 428-3428
>fax + (514) 428-4900