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Hello ABRF,
A collegue has asked me advice on how to purify a membrane protein to
send for mass sequencing. Although the sequence of this protein, Gas1,
is already known from DNA sequencing, it has a
glycosylphosphatidylinosito (GPI) anchor on an unknown residue, which he
wants to determine by the sequencing. His question, which I could not
answer and hope one of you can, was how he could purify the protein,
keeping it in solution, to a level, and in a final solvent, compatible
with mass sequencing. He has already partially purified it by ion
exchange chromatography, but in the presence of a detergent. Another
question was how to remove detergent from an eventually sufficiently
pure protein, while still keeping it in solution.
Thanks for any help, Alex Tossi.
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Dr. Alessandro Tossi
Antiinfective peptides group
Dept. of Biochemistry, Biophysics and Macromolecular Chemistry
University of Trieste
Phone -- 39 40 6763673; Fax -- 39 40 6763691
email: tossi@univ.trieste.it
tossi@icgeb.trieste.it
http://www.univ.trieste.it:80/~nirdbbcm/antimic.html
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