> -----Original Message-----
> From: Association of Biomolecular Resource Facilities
> [mailto:abrf-request@aecom.yu.edu]On Behalf Of alessandro tossi
> Sent: Thursday, February 05, 1998 6:42 AM
> To: Recipients of ABRF List
> Subject: HPLC
>
>
> Hello ABRF,
>
> A collegue has asked me advice on how to purify a membrane protein to
> send for mass sequencing. Although the sequence of this protein, Gas1,
> is already known from DNA sequencing, it has a
> glycosylphosphatidylinosito (GPI) anchor on an unknown residue, which he
> wants to determine by the sequencing. His question, which I could not
> answer and hope one of you can, was how he could purify the protein,
> keeping it in solution, to a level, and in a final solvent, compatible
> with mass sequencing. He has already partially purified it by ion
> exchange chromatography, but in the presence of a detergent. Another
> question was how to remove detergent from an eventually sufficiently
> pure protein, while still keeping it in solution.
>
> Thanks for any help, Alex Tossi.
>