Re: Lys-C digest

Ioannis Papayannopoulos (iap@iname.com)
Thu, 05 Feb 1998 16:15:02 -0500

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If memory serves (I am in the process of moving and not able to look up my
references and notes) EndoLys-C does work in the presence of DTT. It turns
out that EndoLys-C works resepctably, at least for some proteins, at pH as
low as 6 (optimum pH 9), which can be useful if one is concerned about
disulfide scrambling.

Ioannis Papayannopoulos
(Soon to be at) CytoMed, Inc.
Cambridge, MA

At 12:07 2/5/98 -0500, CHARLES MCNEMAR wrote:
>Does anyone know how well Lys-C digests work under reducing conditions?
>
>Charles McNemar, Ph.D.
>Associate Principal Scientist
>Schering-Plough Research Institute
>2015 Galloping Hill Road
>Kenilworth, NJ 07033
>
>908-298-7194
>FAX: 908-298-4844
>
>E-mail: charles.mcnemar@spcorp.com
>
>

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