I tried this once with Wako Lys-C in 10mM DTT with a protein that is
usually digested brilliantly and it was a disaster.
Ken
>Does anyone know how well Lys-C digests work under reducing conditions?
>
>Charles McNemar, Ph.D.
>Associate Principal Scientist
>Schering-Plough Research Institute
>2015 Galloping Hill Road
>Kenilworth, NJ 07033
>
>908-298-7194
>FAX: 908-298-4844
>
>E-mail: charles.mcnemar@spcorp.com
********************************
Ken I. Mitchelhill
The John Holt Protein Structure Laboratory
St. Vincent's Institute of Medical Research
41 Victoria Parade
Fitzroy 3065 Victoria
AUSTRALIA
Telephone: 61-3-9288 2480
Facsimile: 61-3-9416 2676
Email: k.mitchelhill@medicine.unimelb.edu.au
Laboratory: http://www.medstv.unimelb.edu.au/WWWDOCS/SVIMRdocs/JHPSL.html
ABRF: http://www.medstv.unimelb.edu.au/abrf.html
***********************************