On Fri, 6 Feb 1998, Ben Van Der Perre wrote:
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> Dear ABRFers,
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> I have to couple two peptides using Lys residue's ( alpha and epsilon N ).
> These Lys's were inserted in order to couple the N-term of the first
> peptide to the N-term of the second peptide. The first peptide is still
> attached to the resin (Tentagel Sram from Rapp polymere) and the second one
> is already cleaved and purified. I would like to perform the coupling in
> solid phase to avoid different purification steps. Could anyone give me
> some advice on what coupling method should be used, keeping in mind that
> after the coupling the binding should resist the TFA (95 %) cleaving .
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> Thank you
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> Ben Van der Perre.
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