Re: Lys-C digest

Deb McMillen (mcmillen@morel.uoregon.edu)
Fri, 6 Feb 1998 09:58:34 -0800 (PST)

Hi, all,
I use 5 mM DTT with 0.1M Tris pH 9.2, 10% AcCN with Wako Lys-C routinely
with BSA and T4 lysozyme as controls and with my unknowns.

Deb McMillen
Institute of Molecular Biology
University of Oregon
Eugene OR

On Fri, 6 Feb 1998, Ken Mitchelhill wrote:

> Charles,
>
> I tried this once with Wako Lys-C in 10mM DTT with a protein that is
> usually digested brilliantly and it was a disaster.
>
> Ken
>
> >Does anyone know how well Lys-C digests work under reducing conditions?
> >
> >Charles McNemar, Ph.D.
> >Associate Principal Scientist
> >Schering-Plough Research Institute
> >2015 Galloping Hill Road
> >Kenilworth, NJ 07033
> >
> >908-298-7194
> >FAX: 908-298-4844
> >
> >E-mail: charles.mcnemar@spcorp.com
>
>
> ********************************
>
> Ken I. Mitchelhill
> The John Holt Protein Structure Laboratory
> St. Vincent's Institute of Medical Research
> 41 Victoria Parade
> Fitzroy 3065 Victoria
> AUSTRALIA
>
> Telephone: 61-3-9288 2480
> Facsimile: 61-3-9416 2676
>
> Email: k.mitchelhill@medicine.unimelb.edu.au
>
> Laboratory: http://www.medstv.unimelb.edu.au/WWWDOCS/SVIMRdocs/JHPSL.html
> ABRF: http://www.medstv.unimelb.edu.au/abrf.html
>
> ***********************************
>
>