To: ABRF questions
cc:
Subject: LC/MS
Dear ABRF's
I want to run some LC/MS analysis of proteins digest mixtures (<0.8mm id
columns used).
Can anyone advise me which solvent system to use (0.1%TFA vs. 0.1%HCOOH,
....) in order to have the highest sensitivity in UV-analysis and
especially in MS analysis . What with the on-column separation resolution
of peptides when one is changing the ion-pairing reagent? Is there a good
reference article available?
Thanks in advance
Jos Raymackers, Ing.
e-mail (innogenetics) : josray@innogenetics.be
e-mail (private) : jos.raymackers@village.uunet.be
INNOGENETICS NV
Industriepark 7 box 4
9052 Zwijnaarde Ghent
Belgium
tel. (office) : 0032-9-2410778
tel. (lab) : 0032-9-2410775
fax. : 0032-9-2410907