Formic acid is the way to go for the best MS sensitivity. You will
sacrifice resolution on the LC though. You could try post column addition
of formic or propionic acid and use the TFA in your mobile phase. I
believe HP had a recent article on this.
Mike Knierman
knierman@lilly.com
>From: Jos Raymackers@INNOGENETICS on 09/02/98 10:30
>
>
>To: ABRF questions
>cc:
>Subject: LC/MS
>
>
>Dear ABRF's
>I want to run some LC/MS analysis of proteins digest mixtures (<0.8mm id
>columns used).
>Can anyone advise me which solvent system to use (0.1%TFA vs. 0.1%HCOOH,
>....) in order to have the highest sensitivity in UV-analysis and
>especially in MS analysis . What with the on-column separation
resolution
>of peptides when one is changing the ion-pairing reagent? Is there a
good
>reference article available?
>
>Thanks in advance
>
>Jos Raymackers, Ing.
>
>
>
>e-mail (innogenetics) : josray@innogenetics.be
>
>e-mail (private) : jos.raymackers@village.uunet.be
>
>
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