Re: LC/MS
Gary Hathaway (hathaway@cco.caltech.edu)
Mon, 9 Feb 1998 20:29:44 -0700
At 1:30 AM -0700 2/9/98, Jos Raymackers wrote:
>From: Jos Raymackers@INNOGENETICS on 09/02/98 10:30
>
>
>To: ABRF questions
>cc:
>Subject: LC/MS
>
>
>Dear ABRF's
>I want to run some LC/MS analysis of proteins digest mixtures (<0.8mm id
>columns used).
>Can anyone advise me which solvent system to use (0.1%TFA vs. 0.1%HCOOH,
>....) in order to have the highest sensitivity in UV-analysis and
>especially in MS analysis . What with the on-column separation resolution
>of peptides when one is changing the ion-pairing reagent? Is there a good
>reference article available?
>
>Thanks in advance
>
>Jos Raymackers, Ing.
>
Jos,
We have been using Michrom Bioresources' Monitor C18 column with their mix
of HFBA/Acetic acids and acetonitrile/n-pr organic. This gives very
acceptable resolution on-column and low signal suppression. You may wish to
check with Michrom I recall they have a short paper on this. Good luck.
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Gary M. Hathaway, Director
PPMAL - Protein/Peptide Micro Analytical Laboratory
California Institute of Technology
139-74, Division of Biology
Pasadena, CA 91125
http://www.cco.caltech.edu/~ppmal
email Gary: hathaway@caltech.edu
email facility: ppmal@caltech.edu
phone: lab (818) 395-6388 or office (818) 395-2769
FAX (818) 449-3414
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