Sigma has a standard peptide mixture which is good for method development
and column comparison/testing. The Sigma number is H 2016. Met enkephalin
and Leu enkephalin elute very close and are very sensitive to %B
composition on a binary gradient with reversed phase columns. Getting them
to baseline is not too easy if the conditions are not just right. We used
this standard to test performance of HPLC instruments we were considering
for purchase. I can give you the proper gradient settings for an HP,
Waters, or MicroTech if you need.
Regards,
Damon
At 10:20 AM 2/9/98 -0500, you wrote:
>Greetings,
>I wonder if there are available standard sample mixtures for the HPLC
>method development and training which can be used to compare different
>columns and different HPLC separation conditions.
>Thanks,
>Michael Breslav
>R.W. Johnson PRI
>
>
Damon C. Barbacci
Department of Chemistry
Texas A&M University
College Station, TX 77843-3255
phone: (409) 845-0613
fax: (409) 845-9485