Re: The best way to separate glycoproteins
ccarrawa@mednet.med.miami.edu
Tue, 10 Feb 98 07:58:48 EST
Lectin-agarose columns are great for this purpose. The appropriate
lectin must be selected for each glycosylated protein, which can be
done by screening on blots. Common lectins used for this purpose
include Con A and wheat germ agglutinin, but others are also used.
Another consideration is release of the bound glycoprotein with the
appropriate hapten sugar. It is not always best to select the lectin
with the highest avidity of binding, since it will be more difficult
to release the glyprotein.
Good luck!
______________________________ Reply Separator _________________________________
Subject: The best way to separate glycoproteins
Author: Bao-Shiang Lee <boblee@uic.edu> at SMTPMED
Date: 2/10/98 7:25 AM
Hi, everyone, I need some suggestions in separating glycoproteins from
non-glycoproteins. Are there columns that can do the job. I would like to
also separate glycoproteins. Best Wishes. Bob