Re: glycoprotein purification and analysis

ccarrawa@mednet.med.miami.edu
Tue, 10 Feb 98 10:45:54 EST


I came across some old but very basic methods on the use of lectin
affinity method, for those of you interested in glycoprotein
purification and analysis. These are appended in ASCII format.

Cummings RD. Kornfeld S. Fractionation of asparagine-linked oligosaccharides by serial
lectin-Agarose affinity chromatography. A rapid, sensitive, and specific technique. J Biol Chem.
257(19):11235-40, 1982

Cummings RD. Kornfeld S. Characterization of the structural determinants required for the high
affinity interaction of asparagine-linked oligosaccharides with immobilized Phaseolus vulgaris
leukoagglutinating and erythroagglutinating lectins. J Biol Chem. 257(19):11230-4, 1982

Spivak JL. Small D. Hollenberg MD. Erythropoietin: isolation by affinity chromatography with
lectin-agarose derivatives. Proc Natl Acad Sci U S A. 74(10):4633-5, 1977